Outdoor production of floricane-fruiting (FF) blackberry (Rubus subgenus Rubus) is problematic in the Northern Great Plains region of the United States because cane injury and plant death will occur from exposure to temperatures −15 °C and colder. An annual FF blackberry production system using hardwood floricane cuttings would overcome some of the existing limitations of traditional production methods. Several experiments were performed to induce adventitious root formation from one-node hardwood floricane blackberry cuttings taken in winter for the purpose of subsequent growth of a floral shoot. One-node hardwood cuttings of multiple blackberry cultivars (Apache, Arapaho, Kiowa, Osage, Ouachita, Siskiyou, and Triple Crown) were evaluated for rooting potential with and without auxin treatments. Root formation was virtually nonexistent for ‘Apache’, ‘Kiowa’, and ‘Triple Crown’ regardless of the auxin treatment. In general, lower auxin concentrations and the powder formulation produced more roots and had higher root ratings. However, rooting success of cuttings and plant development was low regardless of the rooting method used. Adventitious root production of one-node dormant hardwood FF blackberry cuttings for use in an annual production system had low success regardless of the cultivar, auxin application, rate, and formulation. The variable propagation success rates using single-node hardwood cuttings from ‘Apache’, ‘Arapaho’, ‘Kiowa’, ‘Osage’, ‘Ouachita’, ‘Siskiyou’, and ‘Triple Crown’ plants grown in containers in North Dakota suggested insufficient rooting success for the recommendation of this practice. Additionally, the results suggested these cultivars are not suitable using this method for an annual production system or as a means for large-scale propagation. Although this approach to developing plants from cuttings is of great interest, without a more effective FF blackberry cutting rooting method that can progress through fruit production, an annual blackberry production system in the Northern Great Plains region of the United States is unlikely.
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Abigail R. Debner, Harlene Hatterman-Valenti and Fumiomi Takeda
Mitchell Eicher-Sodo, Robert Gordon and Youbin Zheng
Hydrogen peroxide (H2O2) is an oxidizing agent used to disinfect recirculated irrigation water during the production of organic crops under controlled environmental systems (e.g., greenhouses). To characterize the phytotoxic effects and define a concentration threshold for H2O2, three microgreen species [arugula (Brassica eruca ssp. sativa), radish (Raphanus sativus), and sunflower (Helianthus annuus ‘Black Oil’)], and three lettuce (Lactuca sativa) cultivars, Othilie, Xandra, and Rouxai, were foliar sprayed once daily with water containing 0, 25, 50, 75, 100, 125, 150, or 200 mg·L−1 of H2O2 from seed to harvest under greenhouse conditions. Leaf damage was assessed at harvest using two distinct methods: 1) the percentage of damaged leaves per tray and 2) a damage index (DI). Applied H2O2 concentrations, starting from 25 mg·L−1, increased the percentage of damaged leaves in every species except ‘Black Oil’ sunflower, which remained unaffected by any applied concentration. Symptoms of leaf damage manifested in similar patterns on the surface of microgreen cotyledons and lettuce leaves, while mean DI values and extent of damage were unique to each crop. Fresh weight, dry weight, and leaf area of all crops were not significantly affected by daily H2O2 spray. Identifying how foliar H2O2 damage manifests throughout the crop, as well at individual cotyledon or leaf surfaces, is necessary to establish an upper concentration threshold for H2O2 use. On the basis of the aforementioned metrics, maximum recommended concentrations were 150 mg·L−1 (radish), 100 mg·L−1 (arugula) for microgreens and 125 mg·L−1 (‘Othilie’), 75 mg·L−1 (‘Rouxai’), and 125 mg·L−1 (‘Xandra’) lettuce.
Jaysankar De, Aswathy Sreedharan, You Li, Alan Gutierrez, Jeffrey K. Brecht, Steven A. Sargent and Keith R. Schneider
Cooling procedures used by blueberry (Vaccinium sp.) growers often may include delays up to 24 hours that can damage the fruit through rough handling and adverse temperatures, thereby potentially compromising quality and, subsequently, safety. The objectives of this experiment were to compare forced-air cooling (FAC) compared to hydrocooling without sanitizer (HW) and hydrocooling with sanitizer (HS) regarding the quality and shelf life of southern highbush blueberry [SHB (Vaccinium corymbosum)] and to determine the efficacy of these treatments for reducing Salmonella in SHB. Freshly harvested SHB that were inoculated with a five-serovar cocktail of rifampin-resistant Salmonella were rapidly chilled by FAC or hydrocooling (HW and HS) using a laboratory model system. FAC did not show any significant reduction (P > 0.05) in Salmonella or in the effects on the microbiological quality of blueberries. HW and HS reduced Salmonella by ≈2 and >4 log cfu/g SHB, respectively, on day 0. These postharvest treatments were also evaluated for their ability to help maintain fruit quality throughout a storage period of 21 days at 1 °C. Hydrocooling (both HS and HW) provided more rapid cooling than FAC. Hydrocooled blueberries showed significant weight gain (P < 0.05), whereas FAC resulted in a slight, but insignificant (P > 0.05), reduction in final weight. The results of hydrocooling, both HS and HW, shown in this study could help to extend the shelf life while maintaining or increasing the microbiological quality of fresh market blueberries. Information obtained by this study can be used for developing the best temperature management practices to maintain the postharvest safety and quality of blueberries.
Ryan C. Costello, Dan M. Sullivan, David R. Bryla, Bernadine C. Strik and James S. Owen
New markets for organic northern highbush blueberry (Vaccinium corymbosum L.) have stimulated interest in using composts specifically tailored to the plant’s edaphic requirements. Because composts are typically neutral to alkaline in pH (pH 7 to 8), and blueberry requires acidic soil (pH 4.2 to 5.5), we investigated elemental sulfur (S0) addition as a methodology for reducing compost pH. The objectives were to 1) characterize initial compost chemistry, including the pH buffering capacity of compost (acidity required to reduce pH to 5.0), 2) measure changes in compost chemistry accompanying acidification, and 3) evaluate plant growth and mineral nutrition of blueberry in soil amended with an untreated or acidified compost. Ten composts prepared from diverse feedstocks were obtained from municipalities and farms. Addition of finely ground S0 reduced compost pH from 7.2 to 5.3, on average, after 70 d at 22 °C, and increased the solubility of nutrients, including K (from 22 to 36 mmol(+)/L), Ca (from 5 to 19 mmol(+)/L), Mg (from 5 to 20 mmol(+)/L), and Na (from 6 to 9 mmol(+)/L). Sulfate-S, a product of S0 oxidation, also increased from 5 to 45 mmol(−)/L. The composts were incorporated into soil at a high rate (30% v/v) in a greenhouse trial to evaluate their suitability for use in blueberry production. Shoot and root growth were strongly affected by compost chemical characteristics, including pH and electrical conductivity (EC). Potassium in compost was highly variable (2–32 g·kg−1). Concentration of K in the leaves increased positively in response to compost K, whereas shoot dry weight and root growth declined. Leaf Mg also declined in response to compost K, suggesting that elevated K concentrations in compost may cause Mg deficiency. Composts with the highest K were also high in total N, pH, and EC. Compost acidification to pH ≤ 6 improved growth and increased leaf Mg concentration. On the basis of these results, composts derived from animal manures or young plant tissues (e.g., green leaves) appear to be unsuitable for high-rate applications to blueberry because they usually require high amounts of S0 for acidification and are often high in EC and K, whereas those derived from woody materials, such as local yard debris, appear promising based on their C:N ratio, compost acidification requirement, and EC.
Camilo Escalante-Magaña, Luis F. Aguilar-Caamal, Ileana Echevarría-Machado, Fátima Medina-Lara, Lucila Sánchez Cach and Manuel Martínez-Estévez
Water stress is the main factor responsible for decreased productivity, which affects the growth and development of crops. Plants respond to stress by accumulating compatible solutes, which have a key role in osmotic adjustment, thereby resulting in osmoprotection of the plants. The loss of water can increase the concentration of compatible osmolytes and molecules that regulate the plant metabolism. These solutes can be metabolized as sugars (sucrose, fructose, trehalosa), amino acids (proline), an amphoteric quaternary amine (glycine betaine), and other low-molecular-weight metabolites. However, among all these compatible solutes, proline and glycine betaine occur the most. Proline is an amino acid that can accumulate in low concentrations under optimal conditions; however, stress conditions contribute to its increased content. Few data are available regarding the levels of endogenous glycine betaine on Solanaceae, which is considered a nonaccumulator under water deficit conditions. The objective of this research was to evaluate the role of compatible osmolytes, glycine betaine and proline, in Capsicum sp. plants under different water deficit conditions. In this study, the presence of endogenous levels of proline and glycine betaine in two species of pepper (Capsicum chinense var. Genesis and Rex and Capsicum annuum var. Padron) were found. The concentration levels of proline were 362, 292, and 246 μmol·g−1 DW for Genesis, Rex and Padron respectively, and irrigation conditions (rehydration) of proline levels increased to 381, 395, and 383 μmol·g−1 DW at 21 days. However, glycine betaine levels were 30–70 μmol·g−1 DW. The relative water content, electrolyte leakage, and soil water potential were also analyzed; therefore, the information suggests that proline contributes better to tolerance to water deficit in the genus Capsicum after 14 days of water deficit treatment. It seems that the contribution of glycine betaine is less effective than that of proline; therefore, it does not have an important role in osmotic adjustment.
Harwinder Singh Sidhu, Juan Carlos Díaz-Pérez and Daniel MacLean
Controlled atmosphere (CA) storage has been observed to prolong the shelf life of fresh produce. The objective of this study was to determine whether CA storage performed better than regular air (RA) storage in maintaining fruit quality of six pomegranate (Punica granatum L.) cultivars grown in the state of Georgia. Pomegranate fruit produced in Ty Ty, GA in 2010 and 2011 were stored in CA [5% CO2 + 3% O2, 5 °C, 90% to 95% relative humidity (RH)] or RA (5 °C, 90% to 95% RH) for 3 months. Pomegranate whole fruit and juice were evaluated for various physical and chemical attributes at the end of storage. Fruit differed by cultivar for rind smoothness, fruit cracking, disease incidence, and chilling injury (CI). Fruit stored in CA had a smoother and less shriveled rind, lower CI, fewer disease severity symptoms, and thus better quality than fruit stored in RA. Fruit rind color, total soluble solids (TSS), titratable acidity (TA), and anthocyanin content in fruit juice were unaffected by storage method. The results showed that pomegranate fruit quality was better sustained under CA compared with RA storage.
Jean Carlos Bettoni, Aike Anneliese Kretzschmar, Remi Bonnart, Ashley Shepherd and Gayle M. Volk
The availability of and easy access to diverse Vitis species are prerequisites for advances in breeding programs. Plant genebanks usually maintain collections of Vitis taxa as field collections that are vulnerable to biotic and abiotic stresses. Cryopreservation has been considered an ideal method of preserving these collections as safety back-ups in a cost-effective manner. We report a droplet vitrification method used to cryopreserve 12 Vitis species (Vitis vinifera cvs. Chardonnay and ‘Riesling, V. actinifolia, V. aestivalis, V. jacquemontii, V. flexuosa, V. palmata, V. riparia, V. rupestris, V. sylvestris, V. ficifolia, V. treleasi, and V. ×novae angeliae) using shoot tips excised from plants grown in vitro. Our results demonstrated wide applicability of this technique, with regrowth levels at least 43% for 13 genotypes representing 12 Vitis species. We demonstrated that the droplet vitrification procedure can be successfully replicated by technical staff, thus suggesting that this method is ready for implementation.
Jia Liu, Tingting Xue and Yongbao Shen
Freshly harvested empress tree (Paulownia elongata) seeds have physiologic dormancy. The aim of this study was to investigate the effects of exogenous and endogenous nitric oxide (NO) on the dormancy and germination of empress tree seeds. After treatment with different concentrations of sodium nitroprusside (an NO-releasing compound) solution, the germination percentage of seeds under 12 h of continuous light was significantly greater. Seed germination percentage was promoted significantly by 10–4 M sodium nitroprusside plus cold stratification compared with seeds treated with cold stratification only. At different hours during imbibition, empress tree seeds treated with 2-(4-carboxyphenyl)-4, 4, 5, 5- tetramethylimidazoline -1-oxyl-3-oxide potassium salt (c-PTIO), NG-nitro-L-arginine methyl ester (L-NAME), and sodium tungstate showed reduced seed germination percentages. During the early hours of imbibition, c-PTIO or sodium tungstate treatment inhibited seed germination significantly. The results showed that both exogenous and endogenous NO can release empress tree seed dormancy. Endogenous NO oxide was involved in dormancy release and germination of seeds during the early stages of imbibition. Wider application of NO may be used for breaking seed dormancy in other species.
Robert F. Heyduck, Steven J. Guldan and Ivette Guzmán
In a two-part study, we examined the effect of sowing date and harvest schedule on the yield of spinach (Spinacia oleracea) grown during the winter in 16 × 32-ft-high tunnels in northern New Mexico. Each part of the study was conducted for two growing seasons and took place between 2012 and 2015. In Study A (2012–13 and 2013–14), spinach was sown four times at roughly 2-week intervals (mid-October, early November, mid-November, and early December) and plant density (plants per square foot), plant height (centimeters), and yield (grams per square foot) were measured for three harvests in mid-January, mid-February, and mid-March. The earliest sowing date had the least-dense stands, and plant density increased with each subsequent sowing. The two earliest sowing dates had significantly higher season-long yield than the later two sowings. In Study B (2013–14 and 2014–15), all plots were sown in mid-October, but harvest schedule treatments were staggered such that harvests began at 9, 11, 13, or 15 weeks after sowing and continued at irregular intervals. Treatment 2, with harvests beginning after 11 weeks, had the greatest season-long yield, slightly greater than when harvests began at 9 weeks, and significantly more than when harvest began 13 weeks or later. More importantly, a staggered harvest schedule can provide spinach weekly for direct marketing opportunities.
Ariana P. Torres, Susan S. Barton and Bridget K. Behe
As more individuals use the Internet for business and leisure, the opportunities for firms to promote products and services and to communicate with consumers online increases. The objective of this study was to investigate green industry managerial decisions to engage in online advertising and how much to invest while determining the main drivers contributing to these decisions. A double-hurdle model analyses of 1735 responses to the 2014 National Green Industry Survey, which gathered information on business practices, showed >40% of green industry business invested in online advertising. Typically, businesses investing in online advertising spent more than 43% of all advertising expenditures in online methods, including websites, social media, and newsletters. Furthermore, the decision to engage in online advertising was driven by the percentage of wholesale and contract sales, market access, firm size, product mix, and business owners’ perceptions. Results also showed that the amount of dollars invested in online advertising depended on firm size, tools used to find customers, location, and business owners’ perceptions. Our findings can help extension personnel and policymakers with the design and deliver social media training and educational events. Our findings can also help green industry businesses understand the two-step nature of the decision to invest in online advertising.