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Open access

Dinesh Phuyal, Thiago Assis Rodrigues Nogueira, Arun D. Jani, Davie M. Kadyampakeni, Kelly T. Morgan, and Rhuanito Soranz Ferrarezi

Since the arrival of Huanglongbing (HLB) disease in Florida, several management approaches, including modification of orchard architecture design and nutritional therapy, have been explored. High-density plantings anticipate early economic returns from HLB-affected orchards. With no cure available for HLB, balanced nutrient application through soil and foliar spraying can mitigate the disease. A 2-year study was conducted to investigate the effects of three grapefruit (Citrus paradisi) planting densities [single-row (300 and 440 trees per ha), and double-row high-density (975 trees per ha)], two controlled-release fertilizer (CRF) blends, and foliar-applied micronutrients (FAM) (a blend of B, Mn, and Zn at 0, 1.5, 3, and 6 times the recommended rates) on grapefruit growth and fruit yield, physiological parameters, and foliar nutrient concentrations in an HLB-affected orchard. All the trees tested positive for HLB based on real-time quantitative polymerase chain reaction (qPCR) test. The highest planting density resulted in the lowest trunk diameter and canopy volume. Despite lower yield per tree in 2019–20, 975 trees per ha planting induced the greatest fruit and solid yields per ha. Also, the fruit produced from 975 trees per ha planting tended to be acidic with the deposition of more soluble solids. Use of CRF with higher micronutrients increased canopy volume with the expense of reduced fruit number in 2019–20. FAM did not affect cycle threshold (Ct) value and tree growth parameters. Fruit yield, photosynthesis rate, and stomatal conductance (g S) decreased, and all leaf nutrient concentrations except B increased in 2019–20 with all FAM rates tested. In conclusion, our study showed that high-density planting optimizes yield under HLB-endemic conditions. In addition, supplemental soil and foliar micronutrient application do not enhance yield of HLB-affected trees over a 2-year timeframe, warranting further research for confirmation of results.

Open access

Renae Moran, Jennifer DeEll, and Cindy B.S. Tong

We evaluated regional variation in the Delta Absorbance Meter® index of absorbance difference (IAD) as a measure of harvest maturity and for predicting the occurrence of storage disorders in ‘McIntosh’ apples [Malus ×sylvestris (L.) var. domestica (Borkh.) Mansf.] in 2016 and ‘Honeycrisp’ apples in 2016 and 2017. Apples were grown in Maine (ME), Minnesota (MN), and Ontario (ON), and they were harvested from one orchard in each region, and two to three times each year, followed by cold storage at 0.5 °C for 2 months in 2016 and 4 months in 2017. In 2016, ‘Honeycrisp’ IAD values were similar in ME and ON, but lower than in MN. In 2017, IAD was greater in ME than in the other two regions during the first harvest, and it similar to MN in the latter two harvests and lower in ON than in the other regions. In ‘Honeycrisp’ apples, IAD was more strongly related to starch pattern index (SPI), internal ethylene concentration, and fruit peel blush than to chlorophyll or soluble solids concentration. Soft scald incidence (SSI) of ‘Honeycrisp’ fruit was greater in ME than in MN and ON in both years. In ME, SSI was related to IAD at harvest in both years, but with an inverse relationship with the first harvest and a positive relationship in the second harvest. A positive relationship also occurred in ON in 2017. SSI was not related to IAD at harvest in MN in both years and ON in 2016. Regional similarities in patterns of change in ‘Honeycrisp’ fruit IAD were not consistent from year to year, and this indicates that a single IAD standard should not be used to assess fruit maturity in different regions. In ‘McIntosh’, IAD values were variable among the three regions and were not related to other maturity indicators. IAD was not useful for measuring maturity in ‘McIntosh’ apples, but it was weakly related to core browning incidence.

Open access

Giseiry Rosa-Valentín, Linda Wessel-Beaver, and Jose Carlos V. Rodrigues

One of the most important members of the Potyviridae is Zucchini yellow mosaic virus (ZYMV). It affects watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] as well as other cucurbits in most parts of the world. Although several genotypes have been reported as having resistance to ZYMV, differential responses to ZYMV strains are known to occur. Using a Puerto Rico strain of ZYMV (ZYMV-PR, GenBank accession number MN422959), we tested the response of 11 genotypes [PIs from the U.S. Department of Agriculture (USDA) National Genetic Resources Program] previously reported as having resistance to this virus. In two greenhouse trials, the first three leaves of seedlings of each genotype were mechanically inoculated with ZYMV-PR. An enzyme-linked immunosorbent assay (ELISA) was done on each seedling’s fourth leaf and symptom severity was rated on the first, third, fifth, and seventh leaves. There were significant genotype × trial interactions for most variables, but some genotypes performed consistently in both trials. All seedlings of PI 537277 tested negative for ELISA (absorbance < 0.200) across both trials. PI 537277, PI 595200, PI 595201, and PI 595203 were generally among the accessions with the lowest symptom severity scores. Overall, symptom severity correlated poorly with ELISA readings. But all plants of PI 537277, and most plants of PI 595201 and PI 595203, had low ELISA readings and low severity scores. Despite having low severity scores, PI 595200 was among the genotypes with the highest ELISA readings in trial 2. For the plant breeder, the most useful genotypes are those that exhibit reduced severity as well as low ELISA. PI 537277, PI 595201, and PI 595203 met those criteria in this study. Of these three accessions, PI 595203 would be the most useful in a breeding program because it has shown resistance to the Puerto Rico, Florida, and China strains of ZYMV.

Open access

Pinki Devi, Penelope Perkins-Veazie, and Carol A. Miles

Separately, grafting and the use of plastic mulch can increase yield, quality, and early harvest of watermelon (Citrullus lanatus), especially when plants are under biotic and/or abiotic stress. A 2-year field study was conducted to evaluate the combination of four different rootstocks and two types of plastic mulch (black and clear) on date of watermelon first flowering, fruit ripening, yield, and fruit quality when plants were exposed to Verticillium dahliae. Seedless watermelon cv. Secretariat was grafted onto rootstocks Lagenaria siceraria cv. Pelop, Benincasa hispida cv. Round, and two interspecific hybrid squash rootstocks Cucurbita maxima × C. moschata cvs. Super Shintosa and Tetsukabuto, with nongrafted ‘Secretariat’ as the control. Fruit were harvested 0, 7, and 14 days after both the leaflet and tendril attached to the fruit pedicel were completely dry (fruit considered to be physiologically mature). The area under the disease progress curve (AUDPC) values for verticillium wilt were not different for mulch type in either year, although the overall AUDPC value was greatly reduced in the four grafted treatments (227) compared with nongrafted (743). There was no difference in days to male or female flowering due to mulch type or year, and rootstock did not affect first flowering of male flowers. Female flowering was 14 and 11 days later in 2018 and 2019, respectively, for ‘Secretariat’ grafted onto bottle gourd ‘Round’ compared with ‘Secretariat’ grafted onto ‘Tetsukabuto’. Female flowering of ‘Secretariat’ on ‘Round’ was also 7 days later compared with nongrafted ‘Secretariat’ both years. However, days to first harvest was not different with mulch or rootstock and was 92 days after transplanting (DAT) in 2018 and 114 DAT in 2019. There was no difference in yield (fruit number and weight) due to year, harvest date, or mulch, but there was a difference due to grafting. ‘Secretariat’ grafted onto ‘Super Shintosa’ had the greatest total number and weight of fruit per plant (3.7 and 14.8 kg, respectively), and nongrafted ‘Secretariat’ had the lowest (0.7 and 3.2 kg, respectively). Fruit quality attributes hollow heart formation (rating 3.2/5 on average), hard seed count (6 on average), total soluble solids (11% on average), and lycopene content were not different among mulch type, rootstock treatment, or harvest date; however, lycopene content did differ due to year (52.44 and 32.51 µg·g−1 in 2018 and 2019, respectively). Flesh firmness was highest for watermelon grafted onto ‘Super Shintosa’ rootstock (6.7 N) and lowest for nongrafted watermelon (4.3 N). Overall, rootstocks reduced verticillium wilt severity and increased fruit yield whereas mulch had no effects, and 5 V. dahliae colony forming units (cfu)/g of soil may be the minimum level for impact on watermelon fruit yield.

Open access

Yongxia Zhang, Qingquan Liu, Yinjie Wang, Yongheng Yang, Ting Zhang, Haiying Tong, Wenjie An, Suzhen Huang, and Haiyan Yuan

Open access

Katharina S. Wigg and Irwin L. Goldman

Table beet (Beta vulgaris ssp. vulgaris) is adversely affected by the soilborne fungus, Rhizoctonia solani, which greatly decreases root quality. There are no reports of breeding efforts designed to improve resistance to this fungus in table beet. A collection of table beet PIs, cultivars, and publicly available inbreds were characterized for their response to inoculation with R. solani in three replicated greenhouse experiments conducted between 2017 and 2019. An isolate of AG 2-2 IIIB was used to inoculate 8-week old plants, and both susceptible and resistant sugar beet genotypes were used for comparison. Plants were evaluated for internal and external disease characteristics 3 and 5 weeks postinoculation. Compared with uninoculated controls, PI accessions ranged from 2% to 44% infection, whereas cultivars and inbred lines ranged from 16% to 53% and 22% to 52%, respectively. No commercial cultivar outperformed the resistant sugar beet genotype; however, several PI accessions exhibited lower mean diseased tissue percentages than the resistant sugar beet. This suggests that resistance to R. solani may exist in table beet germplasm. PI accessions exhibited greater resistance on average than did cultivars. Inbred lines exhibited the least amount of variation. Ordinal regression allowed for prediction of infection response, which may be helpful in future selection efforts. ‘Badger Flame’, Ames 22164, PI 502294, PI 169015, ‘Long Season’, ‘Red Cloud’, and ‘Albina Vereduna’ were some of the most resistant accessions in our screens. The findings from this study can be used to inform decisions for breeding for host plant resistance in table beet.

Open access

José Cuenca, Andrés García-Lor, José Juárez, José A. Pina, Luis Navarro, and Pablo Aleza

Open access

Géza Bujdosó, Attila Fodor, and Anita Karacs-Végh

Open access

Jingli Gao, Fengyang Yu, Wenji Xu, Yuee Xiao, and Xiaoying Bi

Open access

Lauren E. Kurtz, Jonathan D. Mahoney, Mark H. Brand, and Jessica D. Lubell-Brand

Feminized hemp seed producers often use selfing to maintain a strain name; however, selfing may lead to inferior plants for cannabidiol (CBD) production. Using three different hemp strains as parents [Candida (CD-1), Dinamed CBD, and Abacus], two outcrosses [Candida (CD-1) × Abacus and Dinamed CBD × Candida (CD-1)] and one self-cross [Candida (CD-1) × Candida (CD-1)] were conducted to produce feminized seed. Progeny from the self-cross were significantly smaller and had less yield than outcrossed progeny. Selfed progeny were variegated and highly variable for total dry weight and floral dry weight. Discriminant analysis of principal components (DAPC) using amplified fragment length polymorphism (AFLP) separated the three progeny populations and showed that outcrossed populations clustered closer to the maternal parent, possibly the result of a maternal effect. Analysis of molecular variance (AMOVA) indicated that most variation (74.5%) was within populations, because the progeny from all three populations are half-siblings of each other. The selfed progeny population had lower expected heterozygosity (He = 0.085) than each of the outcrossed progeny populations (He ≈ 0.10). These results suggest that selfed progeny may demonstrate inbreeding depression resulting from enhanced expression of homozygous recessive traits. It may be beneficial for feminized seed producers to use outcrossing instead of selfing to generate feminized seed for CBD production.