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Open access

Hunter A. Hammock, Dean A. Kopsell, and Carl E. Sams

Light emitting diodes (LEDs) can produce a wide range of narrowband wavelengths with varying intensities. Previous studies have demonstrated that supplemental blue (B) and red (R) wavelengths from LEDs impact plant development, physiology, and morphology. High-pressure sodium (HPS) lighting systems are commonly used in greenhouse production, but LEDs have gained popularity in recent years because of their improved energy efficiency and spectral control. Research is needed to determine the efficacy of supplementary B and R LED narrowband wavelengths compared with traditional lighting systems like HPS in terms of yield, quality, and energy consumption for a variety of greenhouse-grown high-value specialty crops. The objective of this study was to determine the impact of LED and HPS lighting on greenhouse hydroponic basil (Ocimum basilicum var. ‘Genovese’) biomass production and edible tissue nutrient concentrations across different growing seasons. Basil was chosen because of its high demand and value among restaurants and professional chefs. A total of eight treatments were used: one nonsupplemented natural light (NL) control; one HPS treatment; and six LED treatments (peaked at 447 nm/627 nm, ±20 nm) with progressive B/R ratios (10B/90R; 20B/80R; 30B/70R; 40B/60R; 50B/50R; and 60B/40R). Each supplemented light (SL) treatment provided 8.64 mol·m−2·d−1 (100 µmol·m−2·s−1, 24 h·d−1). The daily light integral (DLI) of the NL control averaged 9.5 mol·m−2·d−1 across all growing seasons (ranging from 4 to 18 mol·m−2·d−1). Relative humidity averaged 50%, with day/night temperatures averaging 27.4 °C/21.8 °C, respectively. LED treatments had the greatest total fresh biomass (FM) and dry biomass (DM) accumulation; biomass for LED treatments were 1.3 times greater on average than HPS, and 2 times greater than the NL control. Biomass partitioning revealed that the LED treatments had more FM and DM for the individual main stem, shoots, and leaves of each plant at varying levels. LED treatments resulted in greater height and main stem diameter. Some essential nutrient concentrations were impacted by SL treatments and growing season. An energy analysis revealed that on average, narrowband B/R LED treatments were 3 times more energy efficient at increasing biomass over HPS. LED treatments reduced SL energy cost per gram FM increase by 95% to 98% when compared with HPS. In addition, the rate of electricity consumption to biomass increase varied across LED treatments, which demonstrates that basil uses different B/R narrowband ratios at varying efficiencies. This experiment shows that spectral quality of both supplemental sources and natural sunlight impacts primary metabolic resource partitioning of basil. The application of LED lighting systems to supplement natural DLI and spectra during unfavorable growing seasons has the potential to increase overall biomass accumulation and nutrient concentrations in a variety of high-value specialty crops.

Open access

Arthur Villordon, Jeffrey C. Gregorie, and Don LaBonte

The primary objective of this work was to generate species-specific information about root architectural responses to variations in inorganic phosphate (Pi) availability at the onset of storage root formation among six sweetpotato (Ipomoea batatas) cultivars. Three Pi levels were used: 0 (low Pi); 0.17 (medium Pi); and 0.34 (high Pi) g/pot triple super phosphate (0N–46P–0K). The check cultivar ‘Bayou Belle’ (BB) consistently showed evidence of storage root formation at 15 days in adventitious roots (ARs) grown across three Pi levels and two planting dates (PDs). Storage root formation was also detected in ‘Orleans’ (OR) and ‘Beauregard’ (BX), but it was less consistent relative to BB. In general, BB had the lowest adventitious root (AR) number relative to the other cultivars, but the magnitudes of difference varied with Pi availability and PD. With the first PD, BX had a 45% higher AR number compared with BB in low Pi conditions; however, there were no differences in the second PD. Within cultivars, BX and Okinawa grown in low Pi showed combined 17% and 24% reductions in primary root length (PRL) relative to roots grown in high Pi. BB had a higher lateral root number (LRN) and lateral root density (LRD) across Pi levels, corroborating prior data regarding the association of these root architectural attributes with the onset of storage root formation. The experimental data support the hypothesis regarding the existence of genetic variation for Pi efficiency in sweetpotato and that some well-documented Pi-efficient root traits like high LRN and LRD are indirectly selected for in-breeding programs that focus on early storage root formation and stable yields across environments.

Open access

Richard P. Marini, Tara Auxt Baugher, Megan Muehlbauer, Sherif Sherif, Robert Crassweller, and James R. Schupp

‘Honeycrisp’ (Malus ×domestica) apples were harvested from a total of 17 mid-Atlantic orchards during 2018 and 2019 to verify a previously published bitter pit prediction model. As in the previous study, bitter pit incidence was associated with low calcium (Ca) levels and high ratios of nitrogen (N), potassium (K), and/or magnesium (Mg) to Ca in the fruit peel and excessive terminal shoot growth. The best two-variable model for predicting bitter pit developed with the 2018–19 data set contained boron (B) and the ratio of Mg to Ca (R 2 = 0.83), which is different from previous models developed with data from three individual years (2015–17). When used to predict the bitter pit incidence of the 2018–19 data, our previous best model containing the average shoot length (SL) and the ratio of N to Ca underestimated the incidence of bitter pit. The model is probably biased because one or more important variables related to bitter pit have not yet been identified. However, the model is accurate enough to identify orchards with a low incidence of bitter pit.

Open access

Eric T. Stafne

Since late Mar. 2020, many universities halted normal operations due to the Coronavirus Disease 2019 (COVID-19) pandemic. Although extension uses many different techniques to educate consumers, it has been slow to grasp the power of social media. Faced with a dilemma of using digital methods instead of in-person field days, short courses, and workshops, Twitter was a viable alternative, especially for broad audience engagement. Tweet threads were posted on Twitter every Monday morning from 6 Apr. to 8 June 2020. Each thread consisted of 10 tweets. A hashtag #YardFruits was used to start the thread and for later reference. For the first nine threads only one fruit species was discussed per thread. The final thread consisted of single tweets of several species. Engagement percentage did not differ over time but did differ among the crop species. Tweets that did not include a photo received less engagement (2.7%) than those that did include a photo (4.7%). My Twitter account saw a 6.5% increase in followers during the series. Grape (Vitis sp.), passion fruit (Passiflora sp.), fig (Ficus carica), and pear (Pyrus communis) threads had the least engagement and were different from the Other Fruits thread. All other threads were similar. Extension educators can grow their influence by using well-targeted, focused tweets and tweet threads, especially those that use hashtags and photos.

Open access

Ricardo Goenaga, Angel Marrero, and Delvis Pérez

Dragon fruit (Hylocereus sp. and Selenicereus sp.), also referred to as pitahaya or pitaya, is a member of the Cactaceae family and native to the tropical forest regions of southern Mexico, Central America, and northern South America. Its fruit is becoming increasingly popular as consumers seek healthy and more diverse food products. The crop adapts to different ecological conditions ranging from very dry regions to wet ones receiving more than 3500 mm of rainfall per year. U.S. commercial production of dragon fruit occurs mainly in Florida, southern California, and Hawaii. As growers learn more about this crop and how productive it can be, the acreage planted is likely to increase. Twelve dragon fruit cultivars grown on an Oxisol soil were evaluated for 5 years under intensive management at Isabela, PR. There were significant differences in number and weight of fruit per hectare among years. Cultivars exhibited an increase in fruit number and yield from 2010 to 2013 and then leveled off or declined. There were significant differences among cultivars for number of fruit and yield per hectare. Cultivars N97-17 and N97-15 produced significantly more fruit averaging 74,908 fruit/ha. Significantly higher fruit yield was obtained by cultivars N97-17, N97-20, N97-22, and NOI-13 averaging 17,002 kg·ha−1. Cultivar Cosmic Charlie had the lowest fruit yield, averaging only 25.1 kg·ha−1. Individual fruit weight was significantly higher in cultivars N97-20 and NOI-13 with fruit weight averaging 346.3 g. Cultivars NOI-16, N97-18, and Cosmic Charlie had significantly higher fruit soluble solids than others, averaging 17.4%. Some of the cultivars used in this study have shown horticultural potential and may serve as new planting material for growers.

Open access

Subhankar Mandal and Christopher S. Cramer

Fusarium basal rot (FBR) of onion, which is caused by Fusarium oxysporum f.sp. cepae (Hanzawa) Snyder & Hansen (FOC) results in a substantial loss of marketable bulbs worldwide. One of the main reasons for the lack of FBR-resistant short-day cultivars is the unreliable screening methods available for the mature bulb stage when significant economic damage occurs. The objective of this study was to develop an artificial inoculation method with better quantification of inoculum for an effective selection of FBR-resistant mature onion bulbs. Mature bulbs of seven New Mexican short-day onion cultivars, along with susceptible and tolerant controls, were selected and evaluated for FBR resistance using mycelial and conidial inoculation methods, respectively. Transversely cut basal plates of mature bulbs were inoculated artificially with mycelia or conidia (12 × 105 spores/mL in 2014 and 3 × 105 spores/mL in 2015 embedded in potato dextrose agar plug) of a virulent FOC isolate ‘CSC-515’. Mature bulb evaluation using a visual rating scale (1 = no disease; 9 = >70% basal plate infected) revealed a high degree of FBR severity and incidence irrespective of the genetic background of the cultivars, minimizing the chance of disease escape, which is a significant problem in field inoculation. An attempt to inoculate intact basal plates postharvest resulted in minimal disease development, suggesting that mechanical resistance was conferred by the dry outer layer of the basal plate. The high selection pressure conferred by the conidial inoculation method developed in this study can effectively screen FBR-resistant onion bulbs to replace an unreliable field screening. Concentrations of the conidia lower than 3 × 105 spores/mL are recommended to detect subtle genetic differences in FBR resistance among the onion cultivars and their selected population.

Open access

Larissa Larocca de Souza and Marcelo L. Moretti

Hazelnut (Corylus avellana L.) basal sprouts, or suckers, are removed to train trees as a single trunk, facilitating mechanization. Suckers are routinely controlled with herbicides, often by using nozzles that generate fine droplets and spray volumes as high as 934 L·ha−1, making spray drift a concern. Spray nozzle type and carrier volume can impact herbicide efficacy and drift. Field studies compared the efficacy of 2,4-D and glufosinate in controlling suckers when applied with a flat-fan nozzle, producing fine droplets, to a TeeJet air-induction nozzle, producing ultra-coarse droplets. These nozzles were evaluated at 187 and 374 L·ha−1. Nozzle and carrier volume did not affect the efficacy of 2,4-D based on control, sucker height, or dry weight. The efficacy of glufosinate was unaffected by nozzle type or spray volume in most evaluations. These results indicate that hazelnut suckers can be effectively controlled using drift-reduction nozzles with lower carrier volumes (187 L·ha−1). Drift-reduction nozzles, coupled with lower spray volume, can maintain herbicide efficacy, minimize drift risk, and reduce cost.

Open access

Asmita Paudel, Youping Sun, Larry A. Rupp, and Richard Anderson

Open access

Bin Peng, Jianlan Xu, Zhixiang Cai, Binbin Zhang, Mingliang Yu, and Ruijuan Ma

Peach (Prunus persica) fruit emit more than 100 volatile organic compounds. Among these volatiles, γ-decalactone is the key compound that contributes to peach aroma. The final step in lactones biosynthesis is catalyzed by alcohol acyltransferases (AATs). In this study, five AAT genes were isolated in the peach genome, and the ways that these genes contribute toward the peach aroma were studied. The sequence analysis of the five AATs showed PpAAT4 and PpAAT5 are truncated genes, missing important residues such as HXXXD. The expressions of PpAATs were investigated to identify the roles in creating the peach aroma. The results indicated that only PpAAT1 is highly expressed during γ-decalactone formation. A functional survey of the five PpAATs, using the oleaginous yeast expression system, suggested that only PpAAT1 significantly increased the γ-decalactone content, whereas the other four PpAATs did not significantly alter the γ-decalactone content. Enzyme assays on PpAATs heterologously expressed and purified from Escherichia coli indicated that only PpAAT1 could catalyze the formation of γ-decalactone. All results indicated that PpAAT1 is a more efficient enzyme than the other four PpAATs during the γ-decalactone biosynthesis process in peach fruit. The results from this study should help improve peach fruit aroma.