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Breeding programs around the world continually collect data on large numbers of individuals. To be able to combine data collected across regions, years, and experiments, research communities develop standard operating procedures for data collection and measurement. One such method is a crop ontology, or a standardized vocabulary for collecting data on commonly measured traits. The ontology is also computer readable to facilitate the use of data management systems such as databases. Blueberry breeders and researchers across the United States have come together to develop the first standardized crop ontology in blueberry (Vaccinium spp.). We provide an overview and report on the construction of the first blueberry crop ontology and the 178 traits and methods included within. Researchers of Vaccinium species—such as other blueberry species, cranberry, lingonberry, and bilberry—can use the described crop ontology to collect phenotypic data of greater quality and consistency, interoperability, and computer readability. Crop ontologies, as a shared data language, benefit the entire worldwide research community by enabling collaborative meta-analyses that can be used with genomic data for quantitative trait loci, genome-wide association studies, and genomic selection analysis.
Ginger (Zingiber officinale Rosc.) is a valuable sciophyte crop used as a spice or fresh herb in culinary dishes and for treating medical issues such as osteoarthritis, neurological diseases, vomiting, and asthma. The demand for ginger in the United States is remarkably high; it is produced commercially and exclusively in Hawaii but can only meet ∼20% of US demand. Light for ginger growth may be more important than is often assumed, but the roles of light in ginger growth and rhizome yield are not fully understood. We hypothesized that artificial shade would produce the highest yielding ginger compared with no shade. This study evaluated the impact of different shading suited for ginger growth and rhizome yield of different cultivars grown in a high tunnel. There were three levels (0%, 22%, and 40%) of shade using RCBD design. We analyzed the overall yield (weight) of ginger and the specific yield (weight) of different rhizome parts (biological root, edible root, and seed ginger) per plant in addition to plant growth data. Data were analyzed for 2018 and 2019, and shade conditions influenced ginger growth and yield. There was no significant difference between shade conditions or cultivars, but general trends found that data differed between the two growing seasons. In 2018, Chinese White and Hawaii Yellow produced a better (higher) yield under 0% and 40% shade. However, in 2019, Chinese White and Hawaii Yellow produced the highest total yield under 22% shade, but Bubba Blue produced the highest overall yield at 0% shade.
To investigate factors influencing the seed germination of Sichuan pepper (Zanthoxylum armatum DC.) and determine the optimal germination method, this study used an L16(43) orthogonal test. The effects of compound treatments, including 2.5% sodium carbonate degreasing time, indole acetic acid (IAA) concentration, and IAA soaking time on seed germination were examined. The results indicated that 2.5% sodium carbonate degreasing time was the primary factor affecting the germination rate and vigor index of the seeds. IAA concentration primarily affected the germination index and the duration of germination, whereas IAA soaking time primarily influenced the time lag of germination. In addition, the 2.5% sodium carbonate degreasing time had a significant effect on the germination rate; IAA concentration significantly impacted the germination index; and IAA soaking time had a significant effect on both the germination index and the time lag of germination. Through the analysis and evaluation of the membership function, the optimal treatment combinations for seed germination were determined to be a 24-hour degreasing time with 2.5% sodium carbonate, an IAA concentration of 200 mg·L−1, and an IAA soaking time of 12 hours. This study provides a valuable reference for the future propagation of Zanthoxylum armatum DC.
The objective of this study was to evaluate the effect of fertilizer application and photosynthetic photon flux density (PPFD) on shoot and root growth of chrysanthemum (Chrysanthemum indicum) and begonia (Begonia ×hiemalis) cuttings. During an acclimation phase indoors, unrooted cuttings were treated with a complete fertilizer solution (15N–2.2P–12.5K with micronutrients) that provided 100 mg·L−1 nitrogen or with tap water. Cuttings were placed under PPFDs of 70 or 140 µmol·m−2·s−1 provided by broadband white light-emitting diode fixtures. A finishing phase in a greenhouse was conducted to evaluate carryover treatment effects. Fertilizer application had minimal effects on cuttings during both the acclimation and finishing phases. However, the higher PPFD increased shoot dry weight (DW) in both species and produced shorter chrysanthemum cuttings with almost double the root DW and higher chlorophyll concentration than those under the lower PPFD. During the finishing phase, chrysanthemum cuttings that had been acclimated under the higher PPFD produced 9% and 14% more shoot and root DW, respectively, than those under the lower PPFD, but no treatment differences were measured for begonia. Overall, our results suggest that when there is a fertilizer starter charge present in the substrate, additional fertilizer application during indoor acclimation will not affect shoot and root growth of chrysanthemum and begonia cuttings under conditions similar to those used in our study. Furthermore, cuttings of high-light-requiring species such as chrysanthemum are more likely to benefit from higher PPFD during indoor acclimation than those that require less light such as begonia.
Production of attractive and water-efficient plants native to the plains and prairies of central North America can sometimes be limited because of problems associated with successful rooting of vegetative cuttings. Winecups (Callirhoe involucrata) is an attractive native plant, valued for its long period of bloom, drought tolerance, and winterhardiness, but can be difficult to propagate from seeds and vegetative cuttings. Seed dormancy issues can result in unreliable germination and seedling variations, which reduces crop uniformity. Although propagation from rooted cuttings maintains crop uniformity, cuttings often fail to root or root poorly. Manipulating the growing conditions of stock plants to suppress reproductive growth may improve rooting success of vegetative cuttings. Based on research conducted earlier with other ornamental perennials and programmed photoperiods, winecups plants were grown under three programmed photoperiods to determine if a particular photoperiod could be used to suppress reproductive growth and promote vegetative growth. The study consisted of three experiments, all conducted in similar fashion but at different times during the year. Results indicate that plants grown under 10-hour and 8-hour photoperiods remained vegetative longer when compared with plants grown under 12-hour photoperiod. Vegetative cuttings harvested from plants grown under 8-hour photoperiod had higher percent rooting when compared with vegetative cuttings harvested from plants grown under 10-hour and 12-hour photoperiods. Based on the findings from this research, plant propagators may be able to increase production of winecups by growing stock plants under 8-hour photoperiod.
The underlying therapeutic mechanisms of care farming activities have not been addressed using psychophysiological data. We aimed to understand frontal cortex activation in adults during care farming activities and to explore the psychophysiological effects. Using a randomized crossover study method, 20 adults (average age, 31.3 ± 9.8 years) participated in 10 care farming activities, (four gardening activities, three animal-mediated activities, and three off-farming activities) for 3 minutes each at an actual care farm. Electroencephalography (EEG) was performed during the activity, and emotional states were assessed using subjective emotional questionnaires after each activity. The EEG results revealed that alpha activity increased during gardening activities, such as organizing garden plots and planting plants. Spectral edge frequency 50, which predicts the degree of arousal, increased during interaction with dogs. The results of the subjective emotional questionnaire revealed that participants experienced positive emotions, such as vigor, comfort, naturalness, and relaxation, when participating in care farming activities compared with those while resting. Therefore, this study suggests that care farming activities can induce changes in the frontal cortex activation and positive emotional states in participants. This study elucidates the use of such activities to address mental health-related issues.
Microgreens are young plants used as ingredients and flavoring in various dishes. Their production time is short, and their production methods can be altered to enhance the nutritional content. To date, consumers’ preferences for microgreens that display different esthetic and nonesthetic traits have not been addressed. Additionally, consumers’ perceived risk of production methods used to enhance nutrition has not been investigated. An online survey and choice experiment involving a sample of 821 consumers in Tennessee were performed to investigate these topics. A mixed logit model was used to analyze the data. Both esthetic and nonesthetic traits influenced the consumers’ preferences. Green microgreens were preferred and valued more than light or dark purple microgreens. When fertilizer was used during production to enhance nutrients, consumers perceived the microgreens as riskier to the environment and for personal consumption relative to microgreens with light-induced nutrient enhancement. Using lighting during production to enhance nutrients generated a $0.47 to $0.79 premium relative to no nutrient enhancement. In general, if participants’ perceived risk aligned with the nutrient enhancement attribute (i.e., light, fertilizer), then their willingness to pay for the microgreens decreased. This was amplified for the fertilizer nutrient enhancement attribute more than it was for the light enhancement attribute. In-state production and microgreen height also impacted consumer preferences for microgreens.