āCrimson Cabernetā grape (Vitis vinifera) seeds showed physiological dormancy and germinated at ā¼60% after 60 days of chilling stratification. Fresh seeds harvested after physiological maturity and sown without drying failed to germinate after 30 days when sown on agar. In agar-sown fresh seeds cut at the distal seed end or intact seeds treated with gibberellic acid (GA), the seeds germinated at ā¼20% after 30 days. The highest germination percentages after 30 days were 63% to 83% in fresh, agar-sown seeds that were cut and treated with GA at 5000 mgā Lā1 regardless of stratification time. Similar results were seen in seeds allowed to dry before sowing. Seeds cut and treated with GA at 5000 mgā Lā1 germinated at 79% after 30 days. However, dry seeds sown on germination paper showed lower germination after cutting and GA treatment compared with agar-sown seeds. The highest germination percentages after 30 days in dry, cut seeds on germination paper treated with GA at 2000 and 5000 mgā Lā1 were 33% and 55%, respectively, compared with agar-sown seeds, which germinated at 76% and 79%, with the same treatments. Results from this study provide a system that reduces the need for chilling stratification for grape seed germination by using partial seedcoat removal and GA treatment.
Increasing labor and input costs have driven wine grape (Vitis vinifera) growersā attention to mechanized equipment to assist in vineyard operations. This study evaluates the financial feasibility of investing in vineyard mechanization, in addition to the released intelligent sprayer in hypothetical wine grape vineyards of varying sizes. Our comparative analysis illustrates how mechanization of vineyard practices affects costs and financial metrics. We conducted a costābenefit analysis for seven investment scenarios and examined the economic performance of four metrics. Our findings suggest that investing in a mechanized trimmer is most effective for growers exposed to labor shortages and high wages. A retrofitted intelligent sprayer is superior for reducing input use and associated costs.
Micropropagation of hemp (Cannabis sativa) is constrained by problems with hyperhydricity and culture decline of microshoots. These problems can be reduced by increasing agar and nutrients in the media during micropropagation stages 1 and 2, respectfully. Performance of microshoots of āAbacusā and āWifeā hemp cultured in Driver and Kuniyuki Walnut medium (DKW) for 15 weeks (6 weeks of stage 1 + 9 weeks of stage 2), with subculturing every 3 weeks during both stages 1 and 2, or in Murashige and Skoog with vitamins medium (MS) for 6 weeks (stage 1) followed by Lubell-Brand Cannabis medium (LBC) for 9 weeks (stage 2), with subculturing every 3 weeks during both stages 1 and 2, was evaluated. In a separate study, microshoot performance of āAbacusā and āWifeā in MS for 3 weeks (stage 1) followed by LBC for 6 weeks (stage 2), with subculturing every 3 weeks, using boxes (Magenta GA-7) with lids featuring a vent with a diameter of 10 mm and a pore size of 0.2 ĀµM or using microboxes (Sac O2 O95/114 + OD95) with lids featuring a filter (Sac O2 #10) were evaluated. Shoot multiplication rate (SMR) and explant height were greater for āAbacusā in LBC than DKW. For āWifeā, SMR at 9 weeks was greater in LBC, as LBC provided more nutrients and water than cultures had received in MS initially during stage 1. Culture medium did not influence ex vitro rooting success, which was 75% for āAbacusā and ā„ 90% for āWifeā. Microboxes resulted in greater hyperhydricity of shoots and a lower ex vitro rooting percentage than boxes. For cultivars that are highly prone to developing hyperhydricity, like āAbacusā, the microboxes were not adequate to control this condition.
During the past few years, Americans have experienced a wide variety of stressors, including political tensions, racial/civil unrest, and the coronavirus disease 2019 (COVID-19) pandemic. All of these have led to uncertainty within society. Chronic feelings of helplessness can lead to depression or feelings of hopelessness in those who perceive their situation as unchanging. The purpose of this study was to evaluate the impacts of gardening and outdoor activities during the COVID-19 pandemic on perceptions of hope, hopelessness, and levels of depression, stress, and anxiety. Participants of this study were recruited through online social media platforms such as Facebook and Instagram; 458 participants completed the 21-item Depression Anxiety and Stress Scale inventory as well as the Hope Scale. Our data indicated that individuals who self-reported themselves as gardeners had significantly more positive scores related to levels of stress, anxiety, and depression and a sense of hope. Furthermore, gardeners had lower levels of self-reported depression, anxiety, and stress when compared with those who did not identify themselves as gardeners. The gardeners also had a more positive outlook regarding hope for the future. Additionally, a significant positive correlation was found between the number of hours spent participating in gardening and a sense of hope, and a negative correlation was found between the number of hours gardening and stress levels. Similarly, there was a significant negative correlation between the number of hours spent participating in any outdoor activity and self-reported levels of stress, anxiety, or depression; however, there was a positive correlation between the number of hours spent participating in any outdoor activity and a sense of hope. Our data suggested that more hours spent outside gardening or participating in recreational activities led to less perceived stress, anxiety, and depression and greater levels of hope for the future.
This study examined the interaction between constant liquid fertilization (CLF) concentrations and plant growth regulator (PGR) application concentrations on petunia (Petunia Ćhybrida) growth and flowering in the production and post-production environments. Paclobutrazol application is a common practice in bedding plant production to achieve a compact plant that increases greenhouse space-use efficiency, shipping density, and tolerance to physical handling stresses in the post-production environment. The objective of this research was to determine the best strategy for balancing CLF and PGR application concentration in the greenhouse environment so that growth and flowering can be maximized in the post-production environment. A two-factorial combination of four CLF concentrations [50, 100, 150, or 200 ppm nitrogen (N)] and four paclobutrazol drench concentrations (0, 5, 10, or 20 ppm) were provided to plants during the production phase, and plant growth and flowering were recorded in the production and post-production environments. From a sustainability perspective, the ideal PGR concentration was 5 ppm paclobutrazol, since this concentration resulted in the best combination of production and post-production characteristics and performance. At this PGR concentration, all plant growth and flowering measures increased as CLF increased from 50 to 200 ppm N; however, all CLF concentrations also produced commercially acceptable plants. Therefore, the ideal CLF concentration depends on the size of plant desired; that is, CLF concentrations as low as 50 to 100 ppm N can be provided depending on the market size requirements of the plants being grown. Based on our results, a combination of 50 ppm N CLF with 0 ppm paclobutrazol or 100 ppm N CLF with 5 ppm paclobutrazol both demonstrated adequate growth control during both production and post-production phases.
Threespike goosegrass (Eleusine tristachya) is a difficult-to-control perennial grass of increasing concern for orchard production systems in the Central Valley of California, USA. This grass has a bunch-type growth habit when tillered, which can interfere with orchard operations, particularly nut pickup from the ground at harvest. From 2016 to 2019, herbicide efficacy on threespike goosegrass was evaluated in a walnut (Juglans regia) orchard in Chico, CA, USA; an almond (Prunus dulcis) orchard in Livingston, CA, USA; and a prune (Prunus domestica) orchard in Orland, CA, USA. At each location, two independent experiments were conducted to evaluate 12 preemergent (PRE) herbicide treatments and eight postemergent (POST) treatments over several years, for a total of 16 trials. PRE herbicides were applied in January according to the regionās typical winter orchard management practices. One treatment included an additional sequential application in March to extend residual activity later into the warm season when threespike goosegrass germinates or resumes growth. In separate studies, POST control of established stands of threespike goosegrass was evaluated in May and June of each year. Each trial was conducted in a randomized complete block design with four replications. Threespike goosegrass control was visually estimated monthly for 5 months after the PRE treatments or at weekly intervals for 5 weeks following POST treatments. The most effective PRE treatment was a sequential application of indaziflam in January, followed by a March application of pendimethalin, providing 90% or greater control of threespike goosegrass 5 months after treatment across all sites and all years. Of the POST treatments, the three graminicides outperformed the other treatments with 73% to 91% control overall sites and years at 5 weeks after treatment. Fluazifop had the highest control ratings (85% to 91%) among the graminicide herbicides but was not always statistically better than clethodim or sethoxydim (74% to 83% control). Glyphosate alone resulted in unacceptable control (33% to 51%) regardless of rates tested, experimental sites, or years. Together, these results confirm grower reports of poor glyphosate performance on threespike goosegrass but suggest that effective herbicide programs can be developed to manage threespike goosegrass using PRE herbicides and POST graminicides registered in California orchard crops.
Current economic conditions demonstrate the need for disease-resistant, highly productive pecan (Carya illinoinensis) cultivars for the southeastern United States that can generate high yield potential and can be produced at a reduced cost to enhance grower profit margins. The objectives of this study were to evaluate the performance of the pecan cultivars Lakota, McMillan, and Excel in the humid growing region of the Coastal Plain of Georgia, USA, in the absence of fungicides. In addition, recognizing the excessive nut production of āLakotaā, we evaluated the response of āLakotaā to mechanical fruit thinning. Four trees each of āLakotaā, āMcMillanā, and āExcelā were selected randomly for sampling. Trees within this orchard block receive no fungicide applications. Pecan scab (Venturia effusa) was not observed on any cultivar evaluated throughout the study. Powdery mildew (Microsphaera alni) was observed on all cultivars, but was significantly greater on āMcMillanā in 2020 and 2022 as a result of drier conditions in those years. Based on current data, all three pecan cultivars evaluated during this study are suitable for commercial production; however, āLakotaā and āExcelā performed best under these conditions in terms of nut yield. There was no consistent difference in nut weight among cultivars. āLakotaā has the greatest potential in terms of percent kernel, averaging 58% kernel compared with 49% and 52%, respectively, for āExcelā and āMcMillanā. However, in years of excessive cropping, percent kernel of āLakotaā is reduced significantly. Mechanical fruit thinning increased the following yearās production of āLakotaā by 82% over nonthinned trees.
In the floriculture trade, cut pepper (Capsicum annuum) stems are typically grown for their fruit to add color contrast to the foliage and blossoms of conventional floral arrangements. Stems are commonly stripped of foliage because leaves wilt rapidly. Three divergent plant types and commercial hydration protection spray products were evaluated to identify effective vase life treatments and new pepper lines that combine both fruit and foliar interest with an acceptable postharvest cut stem life. Three inbred US Department of Agriculture pepper breeding lines with a tall vigorous growth habit and black foliage were selected for evaluation as cut stems. Line 190-2 produced upright, tabasco-like fruit; 191-1 produced upright, clustered, round fruit; and 196-1 was fruitless. Three commercial spray treatments Crowning Glory (FLCG), Finishing Touch, and Aqua Finish Clear (AFC) were evaluated on treated cut stems stored at 10 and 23āĀ°C. The pepper breeding line had the greatest influence on cut stem foliage and fruit vase life. The fruitless line, 196-1 exhibited an extended vase life in comparison with fruited lines. Cold storage extended the vase life of cut stems. FLCG reduced foliage vase life at 23āĀ°C, and AFC extended foliage vase life of the fruitless line 196-1. Relative to foliage, fruit exhibited greater resistance to desiccation, with glossier fruit of 191-1 desiccating more rapidly than fruit of 190-2. Similar trends were noted when cut stems were stored at 10āĀ°C for 7 days and moved to 23āĀ°C. However, in 2022 trials, the vase life of 190-2 was shortened, and those of 191-1 and 196-1 were extended, highlighting the influence of preharvest factors on vase life. The results demonstrate that cut stems of new pepper lines with vigorous upright growth habits and black-pigmented foliage, together with diverse fruit morphology, provide innovative possibilities for stunning cut flower arrangements.
A mobile web application called PropG was developed for students to quickly access more than 270 glossary terms defined in a plant propagation textbook. The functionality and usefulness of the app was evaluated by 53 students enrolled in a semester-long online course in plant propagation. Means of pre- and post-test responses to 17 knowledge items showed students perceived a significant knowledge gain in the course for each of the subject categories evaluated. Most students agreed or strongly agreed this learning tool was organized and easy to navigate and would use it in the future. Since 2021, PropG received 153,645 total page views, 90,818 unique visits, and 17,216 returning visits, showing its widespread use in plant propagation.