Summer squash (Cucurbita pepo L.) is a major vegetable crop produced in Georgia and Florida during the fall season. This production is vulnerable to whitefly (Bemisisia tabaci Genn.)-transmitted viruses that lead to severe yield losses. Over the past several years, whitefly populations have increased during the fall, thus leading to an increase in whitefly-transmitted viruses such as Cucurbit leaf crumple virus (CuLCrV) and Cucurbit yellow stunting disorder virus (CYSDV). Whitefly management for summer squash relies on the use of insecticides and can be costly without providing adequate management of the viruses. Deployment of host resistance to whiteflies and their transmitted viruses (CuLCrV and CYSDV) is the best strategy for mitigating yield loss of summer squash; however, no resistant cultivars are commercially available. In the current study, resistance or tolerance to whiteflies, CuLCrV, and CYSDV was determined for squash germplasm from the U.S. Department of Agriculture (USDA) Germplasm Resources Information Network (GRIN), university breeding programs, and commercial companies in Georgia and Florida across 2 years. In both locations and years, visual virus symptom severity scores were collected and a quantitative polymerase chain reaction (qPCR) was used to determine the CuLCrV viral load and CYSDV presence in Georgia. Whitefly-induced feeding damage was evaluated by directly assessing the intensity of silverleaf symptoms and visual counts of whitefly adults on the foliage in the field or in photographs. Virus symptom severity was lower in C. moschata Duchesne ex Poir. genotypes, namely, PI 550689, PI 550692, PI 550694, PI 653064, and Squash Betternut 900, than in other evaluated genotypes. Two C. pepo accessions were common between both locations for viral severity (PI 442294) or viral severity and viral load (PI 171625). Lower CuLCrV loads were identified in C. ecuadorensis Cutler & Whitaker (PI 540895), and C. okeechobeensis (Small) L.H.Bailey (PI 540900) than other evaluated genotypes. Four genotypes tested negative for CYSDV during both years: C. pepo (PI 507882), C. moschata (PI 483345), C. ecuadorensis (PI 390455), and C. okeechobeensis (PI 540900); they are potential sources of resistance. Six C. moschata accessions (PI 211999, PI 550690, PI 550692, PI 550694, PI 634982, and PI 653064) showed high tolerance to silverleaf disorder and had the lowest adult whitefly counts. Collectively, the accessions identified in the current study are potential sources of resistance or tolerance to whitefly and whitefly-transmitted viruses (CuLCrV and CYSDV).
Alexander Luckew, Geoffrey Meru, Ya-Ying Wang, Rodrick Mwatuwa, Mathews Paret, Renato Carvalho, Melanie Kalischuk, Andre Luiz Biscaia Ribeiro da Silva, Joara Candian, Bhabesh Dutta, Rajagopalbabu Srinivasan, Saritha Raman Kavalappara, Naga Charan Konakalla RRD, Sudeep Bag, and Cecilia McGregor
Youping Sun, Genhua Niu, Haijie Dou, Christina Perez, and Lisa Alexander
Hydrangeas are popular landscape plants that are widely grown in many parts of the world. The objective of this study was to evaluate the salinity tolerance of three novel Dichroa ×hydrangea hybrids [Dichroa febrifuga ‘Yamaguchi Hardy’ × Hydrangea macrophylla ‘Hamburg’ (YH × Hamburg), Dichroa febrifuga ‘Yellow Wings’ ×Hydrangea macrophylla ‘Nigra’ (YW × Nigra), and Dichroa febrifuga ‘Yellow Wings’ ×Hydrangea macrophylla ‘Oakhill’ (YW × Oakhill)]. A 52-day greenhouse study was conducted by irrigating container-grown plants with nutrient solution at an electrical conductivity (EC) of 1.1 dS·m−1 (control) or saline solution at an EC of 5.0 dS·m−1 (EC 5) or 10.0 dS·m−1 (EC 10). At harvest, YH × Hamburg and YW × Nigra in EC 5 and EC 10 still exhibited good quality with average visual scores greater than 4.1 (0 = dead; 5 = excellent). For YW × Oakhill, moderate foliar salt damage was observed with an average visual score of 2.9 in EC 5 and 2.2 in EC 10. Compared with control, the shoot dry weight of YH × Hamburg, YW × Nigra, and YW × Oakhill in EC 5 reduced by 35%, 35%, and 55%, respectively, whereas that in EC 10 decreased by 58%, 58%, and 67%, respectively. Elevated salinity also decreased plant height, leaf area, and leaf greenness [Soil Plant Analysis Development (SPAD) readings]; chlorophyll fluorescence (Fv/Fm); performance index (PI); and net photosynthetic rate (Pn). All these responses might result from excess accumulation of sodium (Na+) and chloride (Cl−) ions in hydrangea leaves. In this study, compared with control, leaf Na+ concentration of YH × Hamburg, YW × Nigra, and YW × Oakhill increased 11, 36, and 14 times, respectively, in EC 5, and 31, 53, and 18 times, respectively, in EC 10. Compared with control, leaf Cl− concentration increased 4, 9, and 7 times in EC 5, and 10, 11, and 8 times in EC 10 for YH × Hamburg, YW × Nigra, and YW × Oakhill, respectively. Leaf nitrogen (N), phosphorous (P), potassium (K+), and iron (Fe3+) concentrations decreased at elevated salinity levels but did not cause any nutrient deficiency. In summary, the three Dichroa ×hydrangea hybrids exhibited different salinity tolerance: YH × Hamburg and YW × Nigra were more tolerant than YW × Oakhill. Salt-tolerant hydrangea hybrids should be chosen for landscape use if soil and/or irrigation water are salty.
Rachel A. Itle, Eileen A. Kabelka, and James W. Olmstead
Carotenoids serve as protective antioxidants, and function in normal vision, bone growth, cell division and differentiation, and reproduction. Winter squash (Cucurbita spp.) is an excellent dietary source of carotenoids. The range of colors from yellow to red in Cucurbita species indicates that increasing carotenoid levels through plant breeding is possible. The objective of this research was to determine the heritability of flesh color in winter squash in both Cucurbita moschata Duchesne and Cucurbita pepo L. Segregating families representing F2, BC1P1 and BC1P2 populations were created in two families of C. pepo (‘Table Gold Acorn’ × PI 314806 and ‘Table King Bush’ × PI 314806) and one family of C. moschata (‘Butterbush’ × ‘Sucrine DuBerry’). Broad-sense heritabilities were calculated for the F2, BC1P1, and BC1P2 populations within each of the three families. Heritabilities ranged from 0.19 to 0.82 for L*, 0.28 to 0.97 for chroma, and 0.12 to 0.87 for hue across all families. Transgressive segregation for color space values L* was identified in the ‘Table King Bush’ × PI 314806 C. pepo population. Our results indicate that it is possible to breed for improved flesh color in Cucurbita, but the population size and number of test locations for evaluation need to be increased to provide better heritability estimates. Cucurbita species are grown throughout the world and their availability and low price makes them an important potential source of carotenoids for human nutrition and health for all ages.
Kelly M. Gude, Eleni D. Pliakoni, Brianna Cunningham, Kanwal Ayub, Qing Kang, Channa B. Rajashekar, and Cary L. Rivard
The implementation of high tunnels has shown to increase marketability and/or yield of tomato (Solanum lycopersicum) and lettuce (Lactuca sativa) crops compared with open-field systems. These structures provide the opportunity to alter light intensity and spectral quality by using specific polyethylene (poly) films and/or shadecloth, which may affect microclimate and subsequent crop productivity. However, little is known about how specific high tunnel coverings affect these parameters. The overall goal of this study was to evaluate the impact of various high tunnel coverings on the microclimate and crop productivity of tomato and lettuce. The coverings included standard, ultraviolet (UV)-stabilized poly film (standard); diffuse poly (diffuse); full-spectrum clear poly (clear); UV-A/B blocking poly (block); standard + 55% shadecloth (shade); and removal of standard poly 2 weeks before initial harvest to simulate a movable tunnel (movable). Microclimate parameters that were observed included canopy and soil temperatures, canopy growing degree-days (GDD), and photosynthetic active radiation (PAR), and crop productivity included yield and net photosynthetic rate. Hybrid red ‘BHN 589’ tomatoes were grown during the summer, and red ‘New Red Fire’ and green ‘Two Star’ leaf lettuce were grown in both spring and fall in 2017 and 2018. Increased temperature, GDD, and PAR were observed during the spring and summer compared with the fall. The soil temperatures during the summer increased more under the clear covering compared with the others. For tomato, the shade produced lower total fruit yield and net photosynthetic rate (Pn) compared with the other treatments, which were similar (P < 0.001 and <0.001, respectively). The greatest yield was 7.39 kg/plant, which was produced under the clear covering. For red leaf lettuce grown in the spring, the plants under the clear, standard, and diffuse coverings had significantly greater yield than the movable and shade coverings (P < 0.001). The coverings had less effect on the yield during the fall lettuce trials, which may have been attributed to the decrease in PAR and environmental temperatures. The findings of this study suggest that high tunnel coverings affect both microclimate and yield of lettuce and tomato.
Jules Janick and Harry Paris
In the first century CE, two Roman agricultural writers, Lucius Junius Moderatus Columella and Gaius Plinius Secundus (Pliny the Elder), referred to proto-greenhouses (specularia) constructed for the Emperor Tiberius (42 BCE–37 CE) presumably adjacent to his palace, the Villa Jovis on the Isle of Capri. Pliny stated in Historia Naturalis (Book 19, 23:64) that the specularia consisted of beds mounted on wheels that were moved into the sun, and on wintry days withdrawn under the cover of frames glazed with transparent stone (lapis specularis) to provide fruits of cucumis. According to Pliny, this was “a delicacy for which the Emperor Tiberius, had a remarkable partiality; in fact there was never a day on which he was not supplied it.” The cucumis fruits described by Columella and Pliny, long mistranslated as cucumbers, Cucumis sativus, were in fact long-fruited melons, Cucumis melo subsp. melo Flexuosus Group. They are known today as vegetable melons, snake melons, and faqqous, and were highly esteemed in Rome and ancient Israel.
Modern greenhouses are intensive farming systems designed to achieve high efficiency and productivity. Plants are produced year-round in greenhouses by maintaining the environment at or near optimum levels regardless of extreme weather conditions. Many scientific discoveries and technological advancements that happened in the past two centuries paved the way for current state-of-the-art greenhouses. These include, but are not limited to, advancements in climate-specific structural designs and glazing materials, and temperature control, artificial lighting, and hydroponic production systems. Greenhouse structures can be broadly grouped into four distinct designs, including tall Venlo greenhouses of the Netherlands, passive solar greenhouses of China, low-cost Parral greenhouses of the Mediterranean region, and gutter-connected polyethylene houses of India and African countries. These designs were developed to suit local climatic conditions and maximize the return on investment. Although glass and rigid plastic options are available for glazing, the development of low-cost and lightweight plastic glazing materials (e.g., polyethylene) enabled widespread growth of the greenhouse industry in the developing world. For temperate regions, supplemental lighting technology is crucial for year-round production. This heavily relies on advancements in electro-lighting during the 19th and 20th centuries. The development of hydroponic production systems for the controlled delivery of nutrients further enhanced crop productivity. This article addresses important historical events, scientific discoveries, and technological improvements related to advancements in these areas.
Cary A. Mitchell
The most recent platform for protected horticultural crop production, with the shortest history to date, is located entirely indoors, lacking even the benefit of free, natural sunlight. Although this may not sound offhand like a good idea for commercial specialty-crop production, the concept of indoor controlled-environment plant growth started originally for the benefit of researchers—to systematically investigate effects of specific environmental factors on plant growth and development in isolation from environmental factors varying in uncontrolled ways that would confound or change experimental findings. In addition to its value for basic and applied research, it soon was discovered that providing nonlimiting plant-growth environments greatly enhanced crop yield and enabled manipulation of plant development in ways that were never previously possible. As supporting technology for indoor crop production has improved in capability and efficiency, energy requirements have declined substantially for growing crops through entire production cycles in completely controlled environments, and this combination has spawned a new sector of the controlled-environment crop-production industry. This article chronicles the evolution of events, enabling technologies, and entrepreneurial efforts that have brought local, year-round indoor crop production to the forefront of public visibility and the threshold of profitability for a growing number of specialty crops in locations with seasonal climates.
Austin Brown, James Brosnan, Nicholas Basinger, Wesley Porter, and Gerald Henry
Dallisgrass (Paspalum dilatatum Poir.) control with postemergence herbicides is inefficient and inconsistent from year to year. Control with acetolactate synthase (ALS)-inhibiting herbicides may be enhanced through root absorption, but herbicide movement through dense turfgrass canopies may be difficult. The objectives of this research were to evaluate the influence of verticutting on the postemergence control of dallisgrass and the presence of ALS-inhibiting herbicides within the soil profile. Long-term dallisgrass control [17 weeks after initial treatment (WAIT)] was enhanced in response to verticutting at one of two locations. This may be attributed to differences in turfgrass management (mowing height) before trial initiation that impacted dallisgrass carbohydrate content and herbicide absorption. However, dallisgrass control with certain herbicides was enhanced at the second location in response to verticutting at earlier rating dates. Thiencarbazone + foramsulfuron + halosulfuron (TFH) and trifloxysulfuron at 112 g·ha−1 a.i. and carrier volume of 1628 L·ha−1 (TRI High CV) following mowing + verticutting resulted in the greatest long-term control 17 WAIT at one of two trial locations, 86% and 85%, respectively. Greenhouse experiments confirmed that mowing + verticutting dallisgrass before treatment followed by irrigation led to an increase in herbicide presence within the soil profile, regardless of herbicide. Presence of TFH went from 6.4 to 8.2 mm, trifloxysulfuron at 28 g·ha−1 a.i. and carrier volume of 407 L·ha−1 went from 6.7 to 8.5 mm, and TRI High CV went from 8.6 to 11.8 mm.